Expression and function of ras proto-oncogene proteins in human sperm cells

Expression and function of ras proto-oncogene proteins in human sperm cells.

The presence and role of c-ras proteins were investigated in mature human sperm cells. The v-H-ras monoclonal antibody (mAb) against the c-ras protein, p21, reacted specifically with the acrosomal region of methanol-fixed as well as unfixed-live capacitated and non-capacitated human sperm cell in the indirect immunofluorescence technique. The v-H-ras mAb predominantly recognized c-ras protein o...

RAS proto-oncogene in medullary thyroid carcinoma.

Medullary thyroid carcinoma (MTC) is a rare malignancy originating from the calcitonin-secreting parafollicular thyroid C cells. Approximately 75% of cases are sporadic. Rearranged during transfection (RET) proto-oncogene plays a crucial role in MTC development. Besides RET, other oncogenes commonly involved in the pathogenesis of human cancers have also been investigated in MTC. The family of ...

Sperm - Egg Binding Protein or Proto - Oncogene ?

tized. The hybridization signal of the radiolabeled probe appears as white grains. All specimens were observed under dark field illumination after nuclear counterstain with hematoxylin. Immunostaining for factor Vill-related antigen (4) confirmed that injury was limited to endothelium. 23. Binding was carred out in buffer containing 50 mM NaCI, 5 mM MgCI2, 5% glycerol, 2.5 mM Hepes (pH 7.9), 1 ...

Sperm-egg binding protein or proto-oncogene?

Molecular-genetic analysis of myc and c-Ha-ras proto-oncogene alterations in human carcinoma.

The results of recent studies on the molecular biology of cancer suggest the abnormal expression of cellular proto-oncogenes in the process of carcinogenesis [1]. The activation of proto-oncogenes, i.e., their conversion into cellular oncogenes, is associated with gene structural alterations and mutations resulting in the unregulated production of oncoproteins, matched by deranged biological fu...